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KMID : 0363620130340030126
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Journal of Korean Oriental Medicine
2013 Volume.34 No. 3 p.126 ~ p.142
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Effects of Bogijetong-Tang on Diabetic-peripheral Neuropathy Induced by Streptozotocin in the Mouse
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Lee Jong-Bin
Kim Chul-Jung
Cho Chung-Sik
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Abstract
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Objectives: Previous reports have shown that Bogijetong-Tang (BJT) is effective in peripheral neuropathy induced by taxol and crush injury. In this study, we researched the effects of BJT on diabetic neuropathy induced by STZ in the mouse.
Methods: We performed both in vitro and in vivo experiments to verify the effects of BJT on diabetic neuropathy induced by STZ in mice. Changes in axonal recovery were observed with immunofluorescence staining using NF-200, Hoechst33258, S100β, caspase 3 and anti-cdc2. Proliferation and degeneration of Schwann cells were investigated by immunofluorescence staining and western blot analyses.
Results: BJT showed considerable effects on neurite outgrowth and axonal regeneration in diabetic neuropathy. BJT contributed to the creation of NF-200, GAP-43, Cdc2, phosphovimentin, β1, active β1, β3 integrin, phospho-Erk1/2 protein.
Conclusions: Through this study, we found that BJT is effective for enhanced axonal regeneration via dynamic regulation of regeneration-associated proteins. Therefore, BJT had a pharmaceutical property enhancing recovery of peripheral nerves induced by diabetic neuropathy and could be a candidate for drug development after more research.
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KEYWORD
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Diabetic-peripheral neuropathy, Bogijetong-Tang (BJT), sciatic nerve, Schwann cell, dorsal root ganglia
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